When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

Here, we describe a method of systematic PCR screening with multiround sample pooling for the isolation of rare PCR-positive samples. As an example, we have applied this protocol to the recovery of ...

The polymerase chain reaction (PCR) technique continues to grow and develop, now boasting several different types, having a variety of effective applications such as the detection of pathogens and ...

Association of Notch-1 phenotype with clinical stage progression in non-small cell lung cancer. This is an ASCO Meeting Abstract from the 2014 ASCO Annual Meeting I. This abstract does not include a ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

In the summer of 1984, Henry Erlich, Glenn Horn, Randy Saiki, and myself were working on developing a DNA test for sickle cell anemia (SCA) at Cetus Corp. SCA is an autosomal dominant disease caused ...

With more cases of patients showing clinical symptoms of Covid-19 but RT-PCR tests coming back negative, hospitals have laid down new protocols to stop emerging variants from going undetected. In a ...

While the Delta variant of Covid-19 continues to swirl, many travel companies are cautiously churning back to business, albeit with modified protocols. Cruises are no exception, and Viking enlisted ...