A new method facilitates the automated subcloning of DNA fragments into plasmids. De novo DNA synthesis of an entire construct consisting of a gene (or genes) and a vector is still prohibitively ...
My cry of frustration echoed down the hall. My latest attempt to subclone five important PCR products had failed yet again. After two months of fruitless effort, I was at my wit's end until a ...
In examples 1 and 4 of Figure 1, we examined the target region in the parent molecule after ET recombination to determine whether it had been copied into the subclone, or excised from the parent. In ...
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