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Molecular Cloning_how to put a DNA insert into a plasmid vector
1:16
YouTubeDU Lab
Molecular Cloning_how to put a DNA insert into a plasmid vector
Molecular Cloning: DNA Insertion Summary 1. Cut (Digestion): Use restriction enzymes to open the circular plasmid vector and excise the DNA insert. 2. Paste (Ligation): Mix the cut vector and insert with DNA ligase (usually at a 1:3 molar ratio) to covalently bond them into a single recombinant loop. 3. Transform: Use heat shock or electricity ...
2 views1 day ago
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#cloning
How does cloning work? - explained in 2 mins!
How does cloning work? - explained in 2 mins!
YouTubeSep 7, 2021
The first successful human cloning was performed in 1996.
The first successful human cloning was performed in 1996.
YouTubeMar 17, 2023
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